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A method to improve plant regeneration

A method for the regeneration of plants in vitro has been patented by INRA which is offering a license on the patent or a licensing option with an R&D programme.

Méthode pour améliorer la régénération des plantes. © INRA
Updated on 03/22/2018
Published on 01/31/2018

Scientists have developed a method for the regeneration of plants in vitro which includes at least one step involving the culture of explants displaying altered activity of the mitochondrial respiratory chain (MRC). The invention can be implemented according to one of these MRC inactivation pathways:

  • mutation of a nuclear gene selected from the gene family coding for the PPR proteins involved in MRC assembly and/or activity,


  • addition of an inhibitor of MRC complex I to the culture medium (rotenone).

This method has been developed in Arabidopsis (rotenone and mutations) and proof of concept has been confirmed in the tomato (rotenone) and maize (mutation).


It has been shown that the inhibition of MRC activities enhances the efficiency of regeneration during organogenesis and somatic embryogenesis protocols in monocotyledonous and dicotyledonous species. Such techniques can thus reduce the time and costs involved in plant regeneration (manpower and the scale of experiments) and enable the regeneration of reputedly recalcitrant cultivars.


This method enables:

  • the regeneration of recalcitrant species (such as melon, pea and sunflower);
  • improved regeneration rates and a broadening of the range of regenerant genotypes in species that have already been studied (such as poplar, rice and maize).

It can be used for clonal vegetative multiplication or for the regeneration or transformation of plants.

INRA Transfert Valorisation Manager:
Claire Nodet


  • EP17305782.9

Type of transfer proposed