PVwide Organization

The PVY presents a single-stranded positive sense RNA genome of 10000 bases in length (9703 bases for the PVY^N-605 isolate, Jakab et al., 1997). The 5’-end of the viral genome is covalently linked to the viral encoded VPg protein. The 3’-end is constituted by a polyadenylated sequence. A single Open Reading Frame is present on the PVY genome. This ORF encodes a polyprotein (3063 residues) cleaved by three viral protease (P1, HC-Pro and Nib) to produce nine functional proteins (P1, HC-Pro, P3, 6k1, CI, 6k2, NIa, NIb et CP). These viral proteins are involved in different steps of the viral cycle. The main functions of the PVY encoded proteins are listed below.