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Alanyl-tRNA synthetase (cytosolic and mitochondrial enzyme)

(alanine-tRNA ligase EC 6.1.1.7)

Gene cDNA Protein
Database accessions AC007980 Z22673 SYA_ARATH
FASTA format files gene cDNA cytosolic peptide
mitochondrial peptide

Relationships to other AlaRSs

SYAC_YEAST SYAC_SCHPO SYA_ARATH SYA_BOMMO SYA_HUMAN SYA_ECOLI SYA_HAEIN SYA_THIFE SYA_BARBA SYA_RICPR SYA_HELPY SYA_SYNY3 SYAP_ARATH SYA_THETH SYA_BACSU SYA_MYCTU SYA_MYCGE SYA_MYCPN SYA_METJA SYA_METTH SYA_ARCFU phylogenetic tree

An unnrooted tree from this multiple alignment generated by Clustal X. Clustal X uses a Neighbour Joining algorithm. Terminal branches and branches which occur in 100% of bootstrap repetitions are in black lines and the other less well supported branches in various shades of grey. Plant sequences are indicated in green, other eukaryotic sequences in blue, eubacterial sequences in red and archaebacterial sequences in magenta. Most sequences were obtained from Swissprot.

Subcellular localisation

Our prediction: cytosolic or mitochondrial depending on the AUG used to initiate translation (see Mireau et al. below)

PSORT prediction:
Long peptide: mitochondrial matrix space --- Certainty= 0.685 (see full report)
Short peptide: nucleus --- Certainty= 0.760 (see full report)

MitoProt II predictions:
Long peptide: chloroplast (see full report)
Short peptide: not organellar

ChloroP predictions:
Long peptide: chloroplast (see full report)
Short peptide: not chloroplast (see full report)

 SYAM_ARATH-GFP fusion

References

Mireau H, et al.
The same Arabidopsis gene encodes both cytosolic and mitochondrial alanyl-tRNA synthetases.
Plant Cell. 1996 Jun;8(6):1027-39.

Ge SJ, et al.
An Arabidopsis embryonic lethal mutant with reduced expression of alanyl-tRNA synthetase gene.
Cell Res. 1998 Jun;8(2):119-34.

tRNA & aminoacyl-tRNA synthetase table | taaRSAt home page

Last updated December 31, 1998; Ian Small