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Glycyl-tRNA synthetase (SYG_ARATH; putative cytosolic and mitochondrial enzyme)

(glycine-tRNA ligase EC 6.1.1.14)

Gene cDNA Protein
Database accessions AC008030 AJ002062 O23627
FASTA format files gene cDNA putative cytosolic peptide
putative mitochondrial peptide

Relationships to other GlyRSs

Many eubacterial GlyRSs are ab heterodimers. For these enzymes, in the tree below the two chains were fused before alignment.

SYGA+B_SYNY3 SYGA+B_BACSU SYGA+B_ECOLI SYGA+B_HAEIN SYGP_ARATH SYG_BOMMO SYG_HUMAN SYG_CAEEL SYG_SCHPO SYG_YEAST SYG_ARATH SYG_METJA SYG_MYCGE SYG_MYCPN SYG_THETH SYG_CHLTR SYGA+B_RICPR SYGA+B_HELPY phylogenetic tree

An unnrooted tree from this multiple alignment generated by Clustal X. Clustal X uses a Neighbour Joining algorithm. Terminal branches and branches which occur in 100% of bootstrap repetitions are in black lines and the other less well supported branches in various shades of grey. Plant sequences are indicated in green, other eukaryotic sequences in blue, eubacterial sequences in red and archaebacterial sequences in magenta. Most sequences were obtained from Swissprot.

Subcellular localisation

Our prediction: cytosolic or mitochondrial depending on the AUG used to initiate translation

PSORT prediction:
Long peptide: mitochondrial matrix space --- Certainty= 0.757 (see full report)
Short peptide: chloroplast stroma --- Certainty= 0.551 (see full report)

MitoProt II prediction:
Long peptide: Mitochondrial p(DFM) = 0.91480 (see full report)
Short peptide: PROBABILITY of export to mitochondria: 0.6224 (see full report)

ChloroP predictions:
Long peptide: not chloroplast (see full report)
Short peptide: not chloroplast (see full report)

References

Duchene A-M, Dietrich A
(1997) Isolation and characterization of a glycyl-tRNA synthetase sequence from Arabidopsis thaliana (Accession No. AJ002062).
Plant Physiol. 1997 115: 1730


tRNA & aminoacyl-tRNA synthetase table | taaRSAt home page

Last updated December 31, 1998; Ian Small