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Histidyl-tRNA synthetase (SYHO_ARATH; organellar enzyme)

(histidine-tRNA ligase EC 6.1.1.21)

Gene cDNA Protein
Database accessions ATF12M12 AF020715
FASTA format files gene cDNA peptide

Relationships to other HisRSs

SYH_ECOLI SYH_HAEIN SYH_PORPU SYH_SYNY3 SYH_BACSU SYH_STAAU SYH_RICPR SYH_METJA SYH_METTH SYH_ARCFU SYH_PYRHO SYH_SULSH SYHO_ARATH SYH_HUMAN SYH_MESAU SYHH_HUMAN SYH_FUGRU SYH_CAEEL SYH_ORYSA SYH_ARATH SYH_YEAST SYH_BORBU SYH_TREPA SYH_HELPY phylogenetic tree

An unnrooted tree from this multiple alignment generated by Clustal X. Clustal X uses a Neighbour Joining algorithm. Terminal branches and branches which occur in 100% of bootstrap repetitions are in black lines and the other less well supported branches in various shades of grey. Plant sequences are indicated in green, other eukaryotic sequences in blue, eubacterial sequences in red and archaebacterial sequences in magenta. Most sequences were obtained from Swissprot.

3D Structure

Click on the image/movie to get access to the 3D coordinates of the predicted 3D structure of SYHO_ARATH. If you are using Netscape and have the Chime plug-in installed, you'll get the chance to play with a fully interactive model.

_ARATH SYHO 3Dstructure

Subcellular localisation

Our prediction: mitochondria and plastids, based on results from GFP fusions.

PSORTprediction:
outside --- Certainty= 0.370 (see full report)

MitoProt II prediction:
Mitochondrial: p(DFM) = 0.99382 (seefull report)

ChloroP predictions:
chloroplast (see full report)

 SYHO_ARATH-GFP fusion

References

Akashi K, et al.
Potential dual targeting of an Arabidopsis archaebacterial-like histidyl-tRNA synthetase to mitochondria and chloroplasts.
FEBS Lett. 1998 Jul 10;431(1):39-44.

tRNA & aminoacyl-tRNA synthetase table | taaRSAt home page

Last updated November 18, 2000; Ian Small