From: Psort server <psort@nibb.ac.jp>
Subject: PSORT server: SYL_ARATH PUTATIVE LEURS

=-=-=-=-=-=-= News from the PSORT Server =-=-=-=-=-=-=

November 6, 1996   Minor Version Up (A bug fixed)

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<<Query Information>>

ORIGIN plant
SEQID  SYL_ARATH PUTATIVE LEURS
 MASESKSYAR RDRLLEIEAT VRKWWEDEDV FRAESCENLP KPGEKFFSTF
 PFPYMNGYLH IGHAFSLSKV DFASAYHRLR GANVLLPFGF HCTGMPIKAS
 ADKLRREIEQ FGNPPVFTAE DTTKVPEVQE ESSDTIALPI PGQFKGKKSK
 VAAKAGGQVY QWEIMRSFGL TDSEIANFRE PSEWLYYFPP LAVEDLRAYG
 LGCDWRRSFV TTDVNPFFDA FVRWQMRKLK SMGKIVKDRR YTIFSPLDGQ
 PCADHDRATG EGVQPQEYTL IKMEVVKPFP LKLGPLEGKR VFLAAATLRP
 ETMYGQTNAW VLPDGKYGAY EISETEVFIL TERAALNLAY QNFSKNPQEP
 SCLVELTGYD LIGLPLRSPL SVNEIIYALP MLTILTNKGT GIVTSVPSDA
 PDDYMALQDL IKKPALQDKY GVKTEWLPTE IIPIINIPEF GDKAAEKVCL
 DLKIKSQNDK EKLAEAKRLT YLKGFTEGTM LIGEFFGRKV QEIKPIIKTK
 LIETGEAIIY SEPEKPVMSR SGDECVVALT DQWYITYGES EWRKIAEECL
 SKMNLYSDET RHGFEHTLSW LNQWACSRSF GLGTRIPWDE QFLVESLSDS
 SLYMAYYTVA HIFHDGDMYK GSKSLIRPQQ MNDEVWEYLF CDGPYPKSSD
 IPSAVLSEMK QEFDYWYPLD LRVSGKDLIQ NHLTFFIYNH TALMANRNWP
 RGIRCNGHIM LNSEKMSKST GNFRTLRQSI EEFSATGTRF CLADAGDGVD
 DANFAFETAN AAILRLTKEL TWMEEVLDVE SSLRTGPPST YADKVFENDM
 NIALRLTERA YKDCLFREAL KNGFYDLQAA RDEYRLSCGT GGMHHDLLLK
 FMDVQTRLIV PICPHFADYV WRKVLNKEGC VLTAGWPPSN EPDLVLKSAN
 KYLQDSIVLM RKLLQKQLSG SKKGAKKGAQ VTAVPEGKLK GLVYVNEQFD
 GWRAHCLRIL QSRFDQQTCS FPPDTEMLAE LSATLLQEGK NLKAIQKVCM
 PFLKFKKDEA ISIGTQALNL RLPFGEIEVL QSNKDLIRRQ LGLEEVEIYS
 ASDPDDVSIA GPHASLLTQN PPSPGSPTAI FVTR

<<Result Information>>

PSORT --- Prediction of Protein Localization Sites
                                         version 6.4b(e-mail)
SYL_ARATH          1084 Residues
Species classification: 5

*** Reasoning Step: 1

Preliminary Calculation of ALOM (threshold: 0.5)
     count: 0
McG: Examining signal sequence (McGeoch: modified)
     Length  of  UR:   0
     Peak Value of UR:   1.30
     Net Charge of CR: 2
     Discriminant Score:     -7.78
GvH: Examining signal sequence (von Heijne)
     Signal Score (-3.5): -11.67
     Possible cleavage site: 35
*** Seems to have no N-terminal signal seq.
Amino Acid Composition: calculated from 1
ALOM new cnt: 0 ** thrshld changed to -2
Cleavable signal was detected in ALOM?: 0B
ALOM: finding transmembrane regions (Klein et al.)
     count: 0  value:   1.27  threshold: -2.0
     PERIPHERAL  Likelihood =  1.27
     modified ALOM score:  -1.15
( 3) or uncleavable?
Gavel: Examining the boundary of mitochondrial targeting seq.
     RO motif at: 3
     Uncleavable? Ipos set to: 13
Discrimination of mitochondrial target seq.: 
     notclr ( 0.93)
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Rule: mitochondrial protein
Hydrophobic moment analysis for chloroplast proteins
     Hmax:  8.40 at (68)
Disc.Score from Amino Acid Composition (chloroplast)
     score from the 3-11 region: -0.92
     score from the 1-31 region:  5.16
Chloroplast protein?  Status: negative (-8.12)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)
Res.2 is Ala (a feature for chloroplast seq.)

*** Reasoning Step: 2

KDEL   Count: 0
Checking apolar signal for intramitochondrial sorting
  (Gavel position 13)  from: 64  to: 67  Score:  2.5
Mitochondrial matrix?  Score:  0.36
Checking apolar signal for intrachloroplastic sorting
Howe: Checking the consensus for intrachloropl.sorting
Chloroplast thylakoid memb.?  Score: 0.100
SKL motif (signal for peroxisomal protein): 
     pos: -1(1084), count: 0   
Amino Acid Composition Tendency for Peroxisome:   1.79
Peroxisomal proteins?   Status: notclr
     AAC score (peroxisome): 0.305
Amino acid composition tendency for vacuolar proteins
     Score: -1.31  Status: negative
Checking the amount of Basic Residues (nucleus)
Checking the 4 residue pattern for Nuclear Targeting
Checking the 7 residue pattern for Nuclear Targeting
Checking the Robbins &amp; Dingwall consensus (nucleus)
     Found:  pos: 910 (3) RK LLQKQLSGSK KGAKK
Final Robbins Score (nucleus):  0.60
Checking the RNA binding motif (nucleus or cytoplasm)
nuc modified.   Score:  0.70
Nuclear Signal   Status: notclr ( 0.40)
Checking CaaX motif..
Checking N-myristoylation..
Checking CaaX motif..

----- Final Results -----

                          nucleus --- Certainty= 0.760(Affirmative) 
       mitochondrial matrix space --- Certainty= 0.484(Affirmative) 
           microbody (peroxisome) --- Certainty= 0.305(Affirmative) 
   chloroplast thylakoid membrane --- Certainty= 0.280(Affirmative) 


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