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Threonyl-tRNA synthetase (SYT_ARATH; cytosolic and mitochondrial enzyme)

(threonine-tRNA ligase EC 6.1.1.3)

Gene cDNA Protein
Database accessions AF007270 Y14329 O04630
FASTA format files gene cDNA putative mitochondrial peptide
putative cytosolic peptide
Gene structure

Relationships to other ThrRSs

SYT_MYCGE SYT_MYCPN SYT_METJA SYTC_CAEEL SYTC_HUMAN SYTC_YEAST SYT_ARATH SYTM_YEAST SYT1_BACSU SYT2_BACSU SYT_ECOLI SYT_HAEIN SYT_BUCAP SYT_RICPR SYT_MYCLE SYT_MYCTU SYTP_ARATH SYT_SYNY3 SYT_HELPY phylogenetic tree

An unnrooted tree from this multiple alignment generated by Clustal X. Clustal X uses a Neighbour Joining algorithm. Terminal branches and branches which occur in 100% of bootstrap repetitions are in black lines and the other less well supported branches in various shades of grey. Plant sequences are indicated in green, other eukaryotic sequences in blue, eubacterial sequences in red and archaebacterial sequences in magenta. Most sequences were obtained from Swissprot.

Subcellular localisation

Our prediction: cytosolic or mitochondrial depending on the AUG used to initiate translation

PSORT prediction:
Long peptide: mitochondrial matrix space --- Certainty= 0.920 (see full report)
Short peptide: cytoplasm --- Certainty= 0.450 (see full report)

MitoProt II prediction:
Long peptide: mitochondrial p(DFM) = 0.99794 (see full report)
Short peptide: PROBABILITY of export to mitochondria: 0.0340 (see full report)

ChloroP predictions:
Long peptide: chloroplast (see full report)
Short peptide: not chloroplast (see full report)

References

Ovesna J, et al.
Mitochondrial threonyl-tRNA synthetase, an enzyme specific for a mitochondrially imported tRNA, is likely to be encoded by the same nuclear gene as its cytosolic counterpart in Arabidopsis thaliana.
In: Plant Mitochondria: from Gene to Function (Møller IM et al., eds) Backhuys Publishers, Leiden. pp 139-142.


tRNA & aminoacyl-tRNA synthetase table | taaRSAt home page

Last updated December 31, 1998; Ian Small