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Valyl-tRNA synthetase (SYV_ARATH; cytosolic and mitochondrial enzyme)

(valine-tRNA ligase EC 6.1.1.9)

Gene cDNA Protein
Database accessions U93308 U89986 P93736
FASTA format files gene cDNA mitochondrial peptide
cytosolic peptide
Gene structure

Relationships to other ValRSs

SYV_HELPY SYV_BACSU SYV_LACCA SYV_BORBU SYV_TREPA SYVP_ARATH SYV_ECOLI SYV_HAEIN SYV_SYNY3 SYV_MYCTU SYV_THETH SYV_FUGRU SYV2_HUMAN SYV_NEUCR SYV_YEAST SYVM_ARATH SYV_MYCGE SYV_MYCPN SYV_METJA SYV_METTH SYV_ARCFU SYV_RICPR phylogenetic tree

An unnrooted tree from this multiple alignment generated by Clustal X. Clustal X uses a Neighbour Joining algorithm. Terminal branches and branches which occur in 100% of bootstrap repetitions are in black lines and the other less well supported branches in various shades of grey. Plant sequences are indicated in green, other eukaryotic sequences in blue, eubacterial sequences in red and archaebacterial sequences in magenta. Most sequences were obtained from Swissprot.

Subcellular localisation

Our prediction: cytosolic or mitochondrial depending on the AUG used to initiate translation
The localisation of a GFP fusion translated from the upstream ATG is shown on the right

PSORT prediction:
Long peptide: outside --- Certainty= 0.370 (see full report)
Short peptide: nucleus --- Certainty= 0.980 (see full report)

MitoProt II prediction:
Long peptide: mitochondrial p(DFM) = 0.99253 (see full report)
Short peptide: PROBABILITY of export to mitochondria: 0.0135 (see full report)

ChloroP predictions:
Long peptide: chloroplast (see full report)
Short peptide: not chloroplast (see full report)

 SYVM_ARATH-GFP fusion

References

Zhang JZ, et al.
Suspensor-derived polyembryony caused by altered expression of valyl-tRNA synthetase in the twn2 mutant of Arabidopsis.
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7349-55.

tRNA & aminoacyl-tRNA synthetase table | taaRSAt home page

Last updated November 19, 2000; Ian Small