The fields (column headers) from MSAT database are ...

Chrom. : Chromosome number

Marker : The name of the marker (unique identifier)

BAC/P1 : BAC or P1 containing the locus

Physic Position : The position of the locus in base pairs from the beginning of the chromosome (from the AGI V5 pseudo-molecules,Feb 2004 )

Pattern : The pattern repeated in the microsatellite

Amplif Length Col : The size of the expected PCR fragment (according to the AGI reference sequence)

For each ecotype :         ESL Code

E = PCR Efficiency

        0 : no amplification

        1 : non optimal amplification

        2 : good, regular amplification

 S = PCR Specificity

        NA : when no amplification occured (E=0)

        1 : non-specific amplification (unrelated bands)

        2 : specific amplification

 L = PCR product Length

        for Col : calculated from the sequence

        other ecotypes : estimated on 3% Agarose gel 

              For example :

                E    S    L

              NA NA NA         ... means that this ecotype was not tested for this marker

               0   NA  NA        ... means 'no amplification obtained in these conditions'

               1     2    X           ... is a slightly inefficient amplification still yielding a band of size X

               2     1    X           ... is a 'multi-band' amplification which specific band is of size X

               2     2    X           ... is the best case for genotyping !

Forward Primer 5' -3' : Oligo used to amplify the microsatellite (forward)

Reverse Primer 5' -3' : Oligo used to amplify the microsatellite (reverse)

Polymorphism tested by : People who tested the marker on different accessions; can be "Loudet" (Olivier Loudet from INRA Versailles, olivier.loudet@versailles.inra.fr) or "CNB-INIA" (MT de Andres, JM Martinez-Zapater and C Alonso-Blanco from Dpt de Genetica Molecular de Plantas, CNB-CSIC, Madrid and from Dpt de Biotecnologia, INIA, Madrid)